Journal: Cell Chemical Biology

Article Title: Directed evolution of potent neutralizing nanobodies against SARS-CoV-2 using CDR-swapping mutagenesis

doi: 10.1016/j.chembiol.2021.05.019

Figure Lengend Snippet:

Article Snippet: Post antigen binding, the cells were washed once with ice-cold PBSB and incubated with mouse anti-Myc (1000x dilution) and chicken anti-His (1000x dilution; Invitrogen, PA1-9531) antibodies on ice for 20 min. Post primary incubation, the cells were washed with ice-cold PBSB and incubated with goat anti-mouse IgG AF488 (200x dilution) and donkey anti-chicken IgY F(ab’) 2 fragment AF647 (500x dilution; Jackson ImmunoResearch, 703-606-155) antibodies on ice for 4 min. Post-secondary incubation, the cells were washed once with ice-cold PBSB and evaluated by flow cytometry.

Techniques: Virus, Recombinant, Luciferase, Protease Inhibitor, DC Protein Assay, Plasmid Preparation, Software, Magnetic Beads, Gel Extraction, Purification

Journal: bioRxiv

Article Title: ELP1 Gene Augmentation Restores Visual Function in a Mouse Model of Familial Dysautonomia

doi: 10.64898/2026.01.09.698339

Figure Lengend Snippet: A. Representative retinal flat-mount immunofluorescence images stained for RNA-binding protein with multiple splicing (RBPMS) in P180 control mice and FD mice that were either uninjected or received intravitreal injection (IVI) of AAV2.hELP1 at doses of 5.4 × 10□ vg or 2.7 × 10□ vg. Scale bars, 1 mm (low magnification) and 100 µm (high magnification). B. Quantification of retinal ganglion cell (RGC) density. RGC counts were significantly reduced in FD mice compared with controls and were increased in FD mice treated with either dose of AAV2.hELP1 relative to uninjected FD mice. Cell counts were obtained by enumerating RBPMS-positive cells from four 1 × 1 mm regions per retina. Data are presented as mean ± SEM and were analyzed by one-way ANOVA. Sample sizes for each group are indicated by n within each bar; p < 0.05, p < 0.0001. C. (a,b) Representative immunofluorescence images demonstrating co-localization of Brn3a (green) and human ELP1 (hELP1) in retinal ganglion cells of P30 mice following IVI of AAV2.hELP1 (5.4 × 10□ vg), consistent with expression of the therapeutic transgene in RGCs. Scale bars, 1 mm and 100 µm.

Article Snippet: Select retinas were co-stained with a mouse anti-Brn3a antibody ((Santa Cruz Biotechnology# sc-8429 AF488;1:200) for complementary RGC labeling.

Techniques: Immunofluorescence, Staining, RNA Binding Assay, Control, Injection, Expressing